Standardization of process parameters for the maximum production of extracellular lipase by bacteria, isolated from indigenous sources

Abstract

Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are hydrolases, which act under aqueous conditions on the carboxyl ester bonds present in triacylglycerols to liberate fatty acids and glycerol. Out Of the different lipase sources, bacterial lipase is of considerable economic importance because of potential industrial applications. Efforts to optimize lipase production have been done in different reports in literature but still this field holds a wide scope of research due to different novel types of lipases which are yet to be found out and \ utilized scientifically owing to their characterization and optimum production. In the present study,lipase producing bacteria were screened from water samples around industrial areas near Kolkata and partially characterized. They were used to produce lipase by optimizingmedia components and physicochemical parameters. The growthprofile in production mediashowed that lipase was produced followingnongrowth associated pattern with its maximum activity at 37 °C and pH 7. Lipase production was found to be optimum with an unsaturated oil (olive oil, 10 ml/L) as inducing agentas compared to other oils in media. Lipase activity also increased with the addition of non-ionic detergent like Triton-X 100 upto a certain concentration (3 ml/L).The standardized conditions obtained were as follows: Peptone 10 g/L, yeast extract 3 g/L and MgSO4 0.5 ml/L. The experimental data were validated and 57% increase in lipase production was observedas compared to standard production media described in the literatures.