COMPARATIVE ACTION OF ALTERNATIVE MEDICINES ARSENICUM ALBUM 30CH AND PHOSPHORUS 30CH FOR BALANCING CYTOKINES GENE EXPRESSIONS IN SARS-COV-2 SPIKE PROTEIN INDUCED PATHOLOGICAL CHANGES

Abstract

Alternative medicines may play a significant role both in terms of protective and curative actions against viral diseases like COVID-19. Some clinical trials with alternative medicines against COVID-19 showed controversial outcomes. Therefore, in this study, we explored the efficacy of two such alternative medicines namely Arsenic album 30C and Phosphorus 30C on fertilized chick (Gallus gallus domesticus ) egg model against pathological changes induced by RBD spike protein (S1) antigen of SARS- CoV- 2. In this study, 14th-day-old fertilized eggs were challenged with the S1 protein along with the medicines and different controls. On harvesting after 48 h, the morbid anatomy was observed followed by collection of the allantoic fluid for molecular biology assay. Cytokine gene expressions namely Interleukins – IL -6, IL-8, IL-10, Interferons – IFN α, β, γ; and Transforming growth factor – TGF β1 were studied by RT-PCR along with the housekeeping gene β actin. The results of this study indicated a significant role of these medicines against RBD S1 antigen-induced pathogenic changes in egg models.