http://localhost:80/xmlui/handle/123456789/1059 2026-04-05T15:59:26Z http://localhost:80/xmlui/handle/123456789/10736 Title: Ultra‑Diluted Gelsemium Sempervirens a Known Dna Topoiso‑ merase i (Top i) Inhibitor Exerts Protective Action Against Sars‑Cov‑ 2 Rbd Induced Cytokine Dysregulation Authors: Paira, Krishnendu; Chatterjee, Debasmita; Ghosh, Sayak; Goswami, Pritam; Das, Satadal Abstract: Gelsemium sempervirens (GS) extract is being used in phytomedicine and homeopathy for its anxiolytic properties but its mechanism of action is yet to be understood. Evidence from rodent models suggests existence of its high sensitivity to the central nervous system even in ultra‑diluted conditions. The diverse effects of its extract and/or its main alkaloids‑gelsemine, sempervirine, and koumine have been shown through different experiments in recent years. Sempervirine intercalates with DNA and inhibits topoisomerase‑I activity, which is thought to be a potential target for restricting viral replication during SARS‑CoV‑2 pathogenesis. Delta SARS‑CoV‑2 spike RBD, the recombinant protein, was procured from Abclonal Pvt. Ltd. 14th‑day‑old Gallus gallus domesticus embryos were inoculated with RBD protein along with control alcohol in pre‑ and post‑treatment sets and challenged with Gelsemium 6CH, 30CH and 200CH potencies. After 48h, allantoic ϐluids were collected during harvesting and stored at −20 ℃ for the study of different cytokine gene expressions by RT‑PCR (Reverse Transcription Polymerase Chain Reaction). GS at 6CH, 30CH, and 200CH dilutions showed up‑regulation of IFN‑α and IL‑10 gene expressions in all experimental sets. Tendencies of down‑regulation of the genes were seen with TGF‑β1, IL‑1β, and IL‑6 cytokines, with few exceptions. IFN‑β and IL‑1β gene expression changes were relatively mild and mostly inconclusive. All expressions indicate a possible balancing effect between pro‑inϐlammatory and anti‑inϐlammatory cytokine gene expressions by Gelsemium. 2025-01-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/10735 Title: Advanced in silico design of an optimized multi-epitope peptide vaccine employing immunoinformatics and reverse vaccinology strategies on the model of Listeria monocytogenes Authors: Bhattacharjee, Mainak; Banerjee, Monojit; Mukherjee, Arun Abstract: Listeria monocytogenes is a food-borne pathogen responsible for causing listeriosis with severe consequences in expectant women and immunodeficient individuals due to age, viral infections, or transplants. Despite its alarming mortality rate of 21–50%, there is currently no appropriate medication or protective measure available to prevent the infection in humans. In this context, our current research intends to devise a proficient anti-listeriosis vaccine through thoughtful exploration of reverse vaccinology tools. We examined 368 protein sequences of L. monocytogenes strain CLIP80459 and culled 29 of them as the most potent immunogens. We then followed a stringent subtractive selection strategy to identify 11 cytotoxic T-cell, 9 helper T-cell, and 8 linear B-cell epitopes from the preselected antigens, based on multiple relevant structural, chemical, and immunological features and population coverage. We merged these epitopes using appropriate linkers and included an adjuvant to create the fused peptide vaccine. The physico-chemical and immunological properties of the chimeric peptide were modelled and analyzed, revealing it to be stable, non-toxic, non-allergenic, and highly soluble. Additional investigations involving molecular docking studies followed by molecular dynamics simulation and immune simulation revealed that the designed vaccine is adequately immunogenic and capable of stable, extensive interactions with HLA and TLR2, leading to activation of humoral and cell-mediated immunity. The peptide’s suitability for recombinant expression and simple purification using an E. coli host was demonstrated through in silico cloning studies. Thus, our study led to the development of a preventive yet safe vaccine against listeriosis that awaits wet-lab validation. 2025-04-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/10734 Title: COMPARATIVE ACTION OF ALTERNATIVE MEDICINES ARSENICUM ALBUM 30CH AND PHOSPHORUS 30CH FOR BALANCING CYTOKINES GENE EXPRESSIONS IN SARS-COV-2 SPIKE PROTEIN INDUCED PATHOLOGICAL CHANGES Authors: Debasmita Chatterjee; Krishnendu Paira Abstract: Alternative medicines may play a significant role both in terms of protective and curative actions against viral diseases like COVID-19. Some clinical trials with alternative medicines against COVID-19 showed controversial outcomes. Therefore, in this study, we explored the efficacy of two such alternative medicines namely Arsenic album 30C and Phosphorus 30C on fertilized chick (Gallus gallus domesticus ) egg model against pathological changes induced by RBD spike protein (S1) antigen of SARS- CoV- 2. In this study, 14th-day-old fertilized eggs were challenged with the S1 protein along with the medicines and different controls. On harvesting after 48 h, the morbid anatomy was observed followed by collection of the allantoic fluid for molecular biology assay. Cytokine gene expressions namely Interleukins – IL -6, IL-8, IL-10, Interferons – IFN α, β, γ; and Transforming growth factor – TGF β1 were studied by RT-PCR along with the housekeeping gene β actin. The results of this study indicated a significant role of these medicines against RBD S1 antigen-induced pathogenic changes in egg models. 2024-01-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/10672 Title: Molecular mechanisms of calcium inducing salt tolerance in rice: Ameliorative interaction between CBL4 and P5CR proteins Authors: Singh, Banhishikha; Chatterjee, Anuvab; Chatterjee, Rajeshwari; Chatterjee, Mou; Banerjee, Soma Abstract: The rice plant is sensitive to soil salinity. Calcium (Ca) acts as an ameliorative agent that helps plants induce salt tolerance. This study was carried out with a comparison of the ameliorative effect of calcium on salt-stressed rice seedlings, the determination of the role of salt-responsive protein groups, and the analysis of their genetic expressions in 21-day-old rice seedlings of ten locally cultivable varieties of West Bengal. For this study, 15-day-old seedlings were treated with 200 mM of sodium chloride (NaCl) solutions along with 10 mM of calcium sulfate (CaSO4) treatment. The determination of the relationship between the salt-responsive proteins and the analysis of the gene expression of those corresponding proteins were not carried out earlier on the selected ten locally cultivable rice varieties of West Bengal. The NaCl crystals were visible on the abaxial leaf surface of salt-stressed rice seedlings. The superoxide dismutase activity was increased in rice varieties, and a similar result was also expressed with calcium treatment. The fourier transform infrared spectroscopy-attenuated total reflection spectral result gave strong evidence for the presence of several salt-tolerant proteins and their genetic expression. STRING database results have suggested that the calcium treatment, coupled with the expression of the CBL4 protein, has regulated the P5CR protein of proline biosynthesis for better salt tolerance and osmotic protection. The quantitative real-time polymerase chain reaction and SDS-PAGE gel electrophoresis analysis showed that salt-tolerant varieties, Chinsurah_nona_1, and Jarava had high calcium signaling mechanisms and osmo-protection abilities. Description: DOI: https://doi.org/10.1556/0088.2024.00162 2024-04-02T00:00:00Z